Journal: Oncology Letters

Article Title: microRNA-497 overexpression decreases proliferation, migration and invasion of human retinoblastoma cells via targeting vascular endothelial growth factor A

doi: 10.3892/ol.2017.6083

Figure Lengend Snippet: miR-497 expression level in retinoblastoma tissues and cell lines was determined by reverse transcription-quantitative polymerase chain reaction. (A) miR-497 was significantly downregulated in retinoblastoma tissues compared with normal retinal tissues. (B) The expression level of miR-497 was decreased in Y79 and WERI-Rb-1 retinoblastoma cell lines, in comparison with normal retinal tissues. (C) Following transfection with miR-497 mimics, miR-497 was significantly upregulated in normal retinal tissues or NC. *P<0.01. miR, microRNA; NC, negative control.

Article Snippet: Cell culture The Y79 and WERI-Rb-1 human retinoblastoma cell lines were purchased from the American Type Culture Collection (Manassas, VA, USA).

Techniques: Expressing, Reverse Transcription, Real-time Polymerase Chain Reaction, Comparison, Transfection, Negative Control

Journal: iScience

Article Title: P16 INK4A drives RB1 degradation by UTP14A-catalyzed K810 ubiquitination

doi: 10.1016/j.isci.2024.110882

Figure Lengend Snippet:

Article Snippet: Plasmid: GFP-RB1 , KHAN et al. , Addgene Plasmid #16004.

Techniques: Virus, Recombinant, Lysis, SYBR Green Assay, Transfection, Protease Inhibitor, cDNA Synthesis, Immunoprecipitation, CCK-8 Assay, Cell Cycle Assay, Staining, Mass Spectrometry, Western Blot, Plasmid Preparation, Mutagenesis, Software, Microscopy

Journal: Cell Reports Medicine

Article Title: KRAS G12D -driven pentose phosphate pathway remodeling imparts a targetable vulnerability synergizing with MRTX1133 for durable remissions in PDAC

doi: 10.1016/j.xcrm.2025.101966

Figure Lengend Snippet:

Article Snippet: Monolith NT.115 system (NanoTemper Technologies GmbH, Germany) was used to quantify the interaction of E2F1 (Abcam, #ab82207) and Rb (Proteintech, #Ag11211), p53 (MCE, #HY-P72257) and G6PD (Proteintech, #Ag21862), p53 and E2F1.

Techniques: Mutagenesis, Recombinant, Virus, CCK-8 Assay, Membrane, Concentration Assay, Modification, Transfection, Agarose Gel Electrophoresis, Plasmid Preparation, Extraction, Bicinchoninic Acid Protein Assay, Staining, RNA Extraction, H&E Stain, Viability Assay, Reporter Assay, Labeling, Western Blot, Sequencing, Control, Software

Journal: Diabetes

Article Title: Decreased RB1 mRNA, Protein, and Activity Reflect Obesity-Induced Altered Adipogenic Capacity in Human Adipose Tissue

doi: 10.2337/db12-0977

Figure Lengend Snippet: Effects of transient Rb1 knockdown (~70% reduction for 36 h) in fully differentiated 3T3-L1 adipocytes on the expression of the denoted genes at the mRNA ( A ) and the protein ( B ) level. Treatments: SiC, control siRNA; siRb1, Rb1 siRNA. siRNAs were introduced in the mature adipocytes by electroporation.

Article Snippet: Membranes were immunoblotted with primary antibodies against pThr821 Rb1 and Rb1 (KHO0021 and KHO0011, respectively; Invitrogen); Rb1, FASN, PPARγ, Adipoq, Ucp1, and β-actin (sc-74562, sc-20140, sc-7273, sc-26497, sc-28766, and sc-47778, respectively; Santa Cruz Biotechnology); and Prdm16 (AP18024PU-N; Acris Antibodies).

Techniques: Knockdown, Expressing, Control, Electroporation

Journal: Cell chemical biology

Article Title: Discovery of covalent CDK14 inhibitors with pan-TAIRE family specificity

doi: 10.1016/j.chembiol.2019.02.015

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: RB , Signal Chem , Cat # R05-55G-50.

Techniques: Virus, Recombinant, Kinase Assay, Sequencing, Software